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Image Search Results
Journal: bioRxiv
Article Title: In vivo dissection of Rhoa function in vascular development using zebrafish
doi: 10.1101/2021.03.27.437282
Figure Lengend Snippet: ( A-D ) Whole-mount in situ hybridization analyses of 48 hpf wild type zebrafish embryos probed for rock1 (A,B) or rock2a (C,D) gene expression. (A,C) Lateral views, anterior to left. (B,D) Higher magnification dorsal views of the heads shown in panels A,C. ( E,G,I,K ) Stereoscope transmitted light images of DMSO-treated control sibling (E,I) or 25 μM (G) or 50 μM (K) Rock1/2 inhibitor (Rockout)-treated 50 hpf Tg(kdrl:EGFP), Tg(gata1a:DsRed) embryos. Arrowheads indicate hemorrhage. ( F,H,J,L ) Confocal images of cranial vessels (EGFP-positive, green) and blood cells (DsRed-positive, magenta) in the same embryos shown in panels E,G,I,K. Arrows indicate CtA defects, arrowheads indicate hemorrhage. ( M,N ) Quantitation of the percentage of 52 hpf Rockout-treated (light grey columns) or DMSO-treated (dark grey columns) sibling embryos with cranial hemorrhage (M) or cranial CtA defects (N). * P < 0.0001 compared to DMSO-treated controls, by Cochran-Mantel-Haenszel test. Mean ± SD is shown for each graph.
Article Snippet:
Techniques: In Situ Hybridization, Gene Expression, Control, Quantitation Assay